Oxford Nanopore Sequencing Services

Oxford Nanopore Sequencing Services in Hyderabad — Long-Read WGS, Full-Length 16S/ITS, Plasmid & Metagenomic Workflows

Commercial long-read sequencing and bioinformatics for companies, laboratories and research groups. Choose the Oxford Nanopore workflow that matches your question, then agree sample QC, coverage, analysis and raw-data handover with the scientific team at Nacharam, Hyderabad.

Request a scope & quote →Discuss sample feasibility

Scientist-guided, founder-led
Research and industrial R&D
Nacharam, Hyderabad · India-wide sample enquiries

Commissioned sequencing, with the scope defined first

Long reads answer different questions; choose the right service track.

Commercial sequencing includes accepted samples, a defined run and documented deliverables. It is separate from tuition, internship supervision or a training certificate. Existing DNA and read datasets can also be reviewed for a sequencing-only or analysis-only scope.

Microbial and fungal whole-genome sequencing

Long-read WGS for isolate genomes, structural context and comparative research. DNA integrity, expected genome size, ploidy and reference availability determine the assembly and annotation plan.

Full-length 16S rRNA and ITS

Full-length 16S V1–V9 for bacterial profiling and ITS-based fungal identification or community profiling. Marker choice, primer coverage, reference databases and target organisms set the achievable taxonomic resolution.

Whole-plasmid sequencing

Review entire plasmid constructs, inserts and structural consistency in a suitable workflow that can replace multiple primer-walking reactions. Mixed plasmids, repeats and low input can limit interpretation and must be discussed before the run.

Targeted long-amplicon sequencing

Sequence an agreed long PCR target to inspect sequence identity, variants or linked regions. Primer design, amplification specificity, controls and allele bias are part of the study review.

Rapid pathogen or strain identification

Research-use sequence identification of suitable isolates or defined targets with a scoped priority timeline. Species/strain resolution and detection limits depend on the assay and database; this does not replace clinical diagnosis or statutory certification.

Shotgun metagenomics

Review a mixed-community DNA sequencing plan when taxonomic and gene-content questions extend beyond a marker assay. Required depth, host DNA, expected abundance and compute needs are agreed before accepting a complex sample.

From accepted material to a documented result

A transparent sequencing and analysis handoff.

01
Scientific and sample review
Share the organism or matrix, input state, sample count, intended use and output requirements. Confirm current platform capacity, execution facility, extraction needs, biosafety and a written acceptance checklist.
02
QC, library and run design
Review concentration, purity and DNA integrity, then agree the library preparation, barcoding, target output and controls. Failed QC is reported before further work is authorised.
03
Basecalling and read review
Document basecaller, filtering and demultiplexing settings. Provide read length, yield and quality summaries; report deviations from the agreed data target rather than concealing them.
04
Workflow-specific analysis
Perform the agreed assembly, consensus, marker classification or metagenomic analysis. A plasmid comparison, isolate assembly and community taxonomic table are different deliverables, not interchangeable reports.
05
Scientific interpretation
Review relevant controls, reference limitations, coverage and ambiguity. Findings are framed for the research question and do not promise species-level calls or a complete assembly for every input.
06
Secure data handover
Deliver the agreed raw POD5/FASTQ files, analysis outputs and methods using an approved transfer route. Specify storage volume, retention and download arrangements before sequencing.

Deliverables

Useful files your team can inspect and reuse.

Raw signal and reads

Raw POD5 signal and basecalled FASTQ for the agreed sequencing run, with sample identifiers and file/checksum records. If a client supplies FASTQ only, unavailable raw signal cannot be reconstructed.

Quality reports

NanoPlot-style read-length, yield and quality summaries plus the agreed sample/run QC report. Read quality and reference-dependent measures are described in context.

Consensus or assembled FASTA

Consensus sequences, assembled contigs or plasmid alignments where that analysis is commissioned. Assembly completeness, depth and ambiguous regions are included in the report.

Taxonomic and functional tables

Taxonomy tables for marker/community studies, and gene or pathway tables only where the chosen workflow supports them. Marker-based predictions are labelled separately from shotgun observations.

Publication-grade figures and methods

Agreed plots, legends, analysis parameters and a methods summary support author-led reporting. The investigating team checks scientific conclusions; publication or journal acceptance is not guaranteed.

Optional downstream packages

Genome annotation, comparative analysis, NCBI submission preparation or additional statistical work can be added explicitly. The quotation lists what is included and any separate analysis cost.

Commercial planning

Confirm timing, confidentiality and execution before dispatch.

Typical sequencing-plus-analysis projects are planned over 2–4 weeks after satisfactory QC, with a separate timeline for complex or priority work. “Rapid identification” is a scoped option, not a blanket same-day promise; batching, sample quality and analysis requirements affect delivery.

A mutual NDA and agreed ownership terms can be arranged before confidential information is shared. Project-specific data and agreed results belong to the client under the written contract; third-party software, databases and pre-existing methods retain their own rights. No public sequence deposit is made without approval.

Confirm matrix-specific preparation and transport instructions before sending DNA, cultures or tissue. Local drop-off at Nacharam and remote submissions are accepted only after the material and receiving arrangements are reviewed.

Research-grade scope and clear reporting boundaries.

These Oxford Nanopore services are for non-clinical research, exploratory testing and industrial R&D. Manna Biotech does not represent this work as NABL-accredited testing, a GLP study or patient diagnostic testing. A suspected organism, resistance gene or sequence match is research evidence and must be interpreted within the agreed assay limitations.

Sample suitability, the executing facility, method availability, biosafety, acceptance criteria and deliverables are confirmed in writing before work begins. We do not guarantee a particular result, publication, regulatory approval or commercial outcome.

Scientific leadership

Dr Chathyushya K B

Founder, MD & CEO — PhD Microbiology, ICMR-NIN; PGDM Bioinformatics; Advanced Diploma in Patent Law, NALSAR; 15+ years' scientific experience.

The scientific team reviews the question, sample plan and interpretation so that the report explains what the evidence supports and where further work is needed.

Read the founder's scientific profile →

Request a service quotation

Start with the question your data must answer.

Tell us the sequencing track, organism or sample matrix, number of samples, available DNA/QC, target question, raw POD5/FASTQ and analysis requirements, intended use and timeline.

Send a non-confidential outline first. Do not include patient identifiers or upload proprietary sequence data in this enquiry. Ask for an NDA and an approved transfer route when needed.

Nacharam, Hyderabad laboratory — approximately a 12-minute walk from Habsiguda Metro. Contact us before dispatching samples; collection, preservation and transport instructions depend on the approved assay.

Contract and bulk enquiries: +91 8977681985 · Technical scope: +91 8978792215

Related services

Choose the service that matches the scientific question.

Detailed WGS service scope

Review organism-specific extraction, assembly, annotation, AMR screening and genome data handover.

16S, ITS and microbiome profiling

Plan marker selection, sample matrices, controls and community analysis deliverables.

Nanopore training for students

Use the separate training route for hands-on instruction and supervised learning.

Analysis-only support

Discuss authorised existing sequence data, methods, figures and interpretation requirements.

Aqua PCR screening

Consider a targeted pathogen assay when a specific shrimp or fish disease question needs a focused test.

Questions

Frequently asked questions

Which Nanopore service should we choose for a genome, plasmid or mixed community?

Choose WGS for an isolate genome, whole-plasmid sequencing for a construct, full-length 16S/ITS for an agreed microbial marker question, or shotgun metagenomics for broader community DNA content. A targeted long amplicon may be more appropriate for one region. We review the objective and input before recommending a track; the most extensive assay is not always the most useful.

Can whole-plasmid sequencing replace primer walking?

For suitable plasmid preparations, long-read whole-plasmid sequencing can replace multiple primer-walking reactions and help review insert sequence and structural arrangement. Mixed populations, contamination, repeats and insufficient read support can prevent an unambiguous answer. The analysis scope specifies reference comparison, consensus requirements and limitations; perfect closure is not guaranteed.

Will we receive raw POD5/FASTQ and the complete analysis report?

For a commissioned sequencing run, the written scope lists raw POD5 and FASTQ delivery, QC reports and the appropriate consensus/assembled FASTA, taxonomic tables or other outputs. Agree transfer size and retention before the run. An analysis-only engagement can return only the raw inputs supplied by the client, not missing POD5 signal. Additional annotation or functional analysis must be included explicitly.

Can you provide rapid pathogen identification or patient diagnostics?

Priority research identification can be considered after review of the material, assay, database and current schedule. A species-level or strain-level answer is not assured for every sample, and sequencing turnaround is confirmed in writing. This service is non-clinical and is not patient diagnosis, a treatment recommendation or a statutory pathogen clearance certificate.

Is this a paid commercial service or a student training programme?

This route is for commissioned sequencing and bioinformatics under a service scope and quotation. Students seeking practical teaching should use the Nanopore sequencing training page. Academic investigators may commission research work here with institutional approvals and author-led interpretation. An NDA, data ownership and project-specific turnaround can be agreed before confidential details or samples are transferred.